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The crude was further diluted with water (9 mL) and purified by HPLC (eluent A 0.1% TFA in water, eluent B 0.1% TFA in acetonitrile/water 4/1 v/v, 50 °C, detection at 215 nm, 6 mL min −1 , 0–20% eluent B in 5 min, then 20–45% eluent B in 60 min, C18XBridge column) to give the cyclic peptide cK1-1 as a white solid after lyophilization (1.71 mg, 31%)