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Following clearance by centrifugation, protein concentration of lysates was determined using a Pierce BCA protein assay (23225, Fisher Scientific) and 30 μg of lysate was prepared using 4× LDS sample buffer (Thermo Fisher) and 10% 2-mercaptoethanol and run on NuPAGE 4–15% Bis–Tris gels (Thermo Fisher)
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The fraction of macrophages that transformed into lipid-associated macrophages ranged from ∼70-90%
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